Mario Mandzukic suffered a sprained left ankle during training on Monday and has therefore been left out of the 20-man squad travelling to England.
The Serie A champions are otherwise mostly at full strength, with Emre Can the only other major absentee as he recovers from a thyroid problem.
Juve head into the match at Old Trafford as leaders of Group H, having claimed six points from a possible six this season. United are two points further back in second place.
Juve squad for United: Szczesny, Pinsoglio, Perin; De Sciglio, Chiellini, Benatia, Pjanic, Ronaldo, Dybala, Douglas Costa, Alex Sandro, Matuidi, Barzagli, Cuadrado, Kean, Bonucci, Cancelo, Rugani, Bentancur, Bernardeschi