After Jordi Alba left the pitch in tears, Gerard Pique followed after, both limping off the pitch in the UCL encounter against City.
The tearful fullback appears to have worsened a muscular problem he complained of while on international duty.
Pique, on the other hand, couldn’t continue after failing to shake off the effects suffered from a clash with David Silva. Digne and Matthieu replaced both defenders respectively.
Alba’s injury is a further blow for Barcelona boss Luis Enrique, who is already facing a shortage of bodies in the full-back positions.
Sergio Roberto missed the City clash with an injury and with Aleix Vidal out of favour, Javier Mascherano was forced to start at right-back.